Huovilainen A, Kinnunen L, Pöyry T, Laaksonen L, Roivainen M, Hovi T
Department of Viral Diseases, National Public Health Institute, Helsinki, Finland.
Virology. 1994 Feb 15;199(1):228-32. doi: 10.1006/viro.1994.1116.
The Saukett/USA/50 strain is the type 3 component of the inactivated poliovirus vaccine. The capsid-coding region of genomic RNA of Saukett strains from five different sources was sequenced and the sequence differences were correlated with antigenic differences measurable with poliovirus type 3-specific neutralizing monoclonal antibodies. All strains appeared to have capsid protein genes identical in size to those of the entirely sequenced type 3 poliovirus strains. The nucleotide sequence identity between the strains was 91% on the average and the strains could be divided into three groups. Amino acid differences were seen in 30 positions located throughout the capsid region both within and outside the known antigenic sites. Substitutions at the known antigenic sites explained most of the observed antigenic differences. Use of the atomic coordinates of the crystal structure model of the Sabin 3 virus and prior data based on escape mutants and peptide scanning revealed that most of the exposed substitutions located outside the known antigenic sites are spatially associated with regions found to be antigenic by either or both of these methods.
索基特/美国/50株是灭活脊髓灰质炎病毒疫苗的3型组分。对来自五个不同来源的索基特株基因组RNA的衣壳编码区进行了测序，并将序列差异与用3型脊髓灰质炎病毒特异性中和单克隆抗体可测量的抗原差异相关联。所有毒株的衣壳蛋白基因大小似乎与完全测序的3型脊髓灰质炎病毒毒株相同。这些毒株之间的核苷酸序列同一性平均为91%，且可分为三组。在衣壳区域内已知抗原位点内外的30个位置观察到氨基酸差异。已知抗原位点的替换解释了大部分观察到的抗原差异。利用萨宾3病毒晶体结构模型的原子坐标以及基于逃逸突变体和肽扫描的先前数据表明，位于已知抗原位点之外的大多数暴露替换在空间上与通过这两种方法之一或两者发现具有抗原性的区域相关。
