Chambon M, Jallat-Archimbaud C, Bailly J L, Gourgand J M, Charbonne F, Henquell C, Fuchs F, Peigue-Lafeuille H
Laboratoire de Bactériologie-Virologie, Faculté de Médecine et Pharmacie, Clermont-Ferrand, France.
Appl Environ Microbiol. 1997 Aug;63(8):3199-204. doi: 10.1128/aem.63.8.3199-3204.1997.
Significant intratypic differences in the glutaraldehyde (GTA) sensitivity of echovirus isolates have been shown. While exploring ways to optimize the study of GTA sensitivity of enteroviruses, we also observed intratypic differences in poliovirus type 1 isolates collected in France. A suspension procedure was used for assessing the virucidal effect of GTA at low concentrations (< or = 0.10%) against purified viruses. Two recent isolates of poliovirus type 1 tested were first fully characterized by the PCR restriction fragment length polymorphism (RFLP) test. The RFLP pattern of clinical isolate 5617 was similar to that of poliovirus type 1 LS-c, 2ab (Sabin strain), confirming the vaccine origin of strain 5617. The RFLP pattern of strain 5915 recovered from sewage was different from that of the Mahoney strain, suggesting a genetic variation in this wild isolate. We then analyzed under the same controlled conditions the GTA sensitivities of both isolates and their respective prototype strains. The wild Mahoney and 5915 strains exhibited significantly lower sensitivities to GTA than did the vaccine Sabin and 5617 strains. The inactivation rates of clinical isolates 5617 and 5915 were very similar to those of their corresponding reference Sabin and Mahoney strains. Both the conformational structure of the capsid of each strain and the amino acid constitution of structural polypeptides could be involved in the variations observed. The relevance of our comparative sensitivity studies to standardization of virucidal tests is discussed.
已表明肠道病毒分离株对戊二醛（GTA）的敏感性存在显著的型内差异。在探索优化肠道病毒GTA敏感性研究方法的过程中，我们还观察到在法国收集的1型脊髓灰质炎病毒分离株存在型内差异。采用一种悬浮程序来评估低浓度（≤0.10%）GTA对纯化病毒的杀病毒效果。首先通过PCR限制性片段长度多态性（RFLP）试验对所检测的两株近期1型脊髓灰质炎病毒分离株进行了全面鉴定。临床分离株5617的RFLP图谱与1型脊髓灰质炎病毒LS-c、2ab（Sabin株）相似，证实了5617株的疫苗来源。从污水中分离出的5915株的RFLP图谱与Mahoney株不同，表明该野生分离株存在基因变异。然后，我们在相同的控制条件下分析了这两株分离株及其各自原型株对GTA的敏感性。野生的Mahoney株和5915株对GTA的敏感性显著低于疫苗株Sabin和5617株。临床分离株5617和5915的灭活率与其相应的参考株Sabin和Mahoney株非常相似。每个毒株衣壳的构象结构和结构多肽的氨基酸组成都可能与所观察到的变异有关。本文讨论了我们的比较敏感性研究与杀病毒试验标准化的相关性。
