枯草芽孢杆菌马堡菌株30S和50S活性核糖体亚基的分离
Isolation of 30S and 50S active ribosomal subunits of Bacillus subtilis, Marburg strain.
作者信息
Guha S, Szulmajster J
出版信息
J Bacteriol. 1975 Dec;124(3):1062-6. doi: 10.1128/jb.124.3.1062-1066.1975.
Abstract
Active 30S and 50S ribosomal subunits were isolated from Bacillus subtilis. These subunits were able to perform not only protein synthesis in the presence of artificial or natural messenger ribonucleic acid but also the specific functions characteristic of each of the subunits. Thus the 30S subunits alone are able to bind formyl-methionyl-transfer ribonucleic acid, and the 50S subunits carry the peptidyl transferase activity.
摘要
从枯草芽孢杆菌中分离出活性30S和50S核糖体亚基。这些亚基不仅能够在人工或天然信使核糖核酸存在的情况下进行蛋白质合成，还能执行每个亚基特有的特定功能。因此，仅30S亚基就能结合甲酰甲硫氨酰转移核糖核酸，而50S亚基具有肽基转移酶活性。
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