Takeyama M, Matsuo H, Mori K
Department of Clinical Pharmacy, Oita Medical University, Japan.
Chem Pharm Bull (Tokyo). 1993 Dec;41(12):2197-9. doi: 10.1248/cpb.41.2197.
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a gastrin-like immunoreactive substance (G-IS) in human plasma was developed. For competitive reactions, the gastrin antibody was incubated with gastrin standard (or sample) and beta-D-galactosidase labeled synthetic C-terminal gastrin I fragment (residue 2-17). Free and antibody-bound enzyme hapten were separated using an anti-rabbit IgG coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 1 to 20 fmol/ml (2.1 to 42 pg/ml) of gastrin. The levels of G-IS determined in human plasma were 7.8 +/- 1.6 pg/ml before lunch and 26.4 +/- 8.4 pg/ml after lunch.
已开发出一种用于检测人血浆中胃泌素样免疫反应性物质（G-IS）的灵敏且特异的双抗体酶免疫测定法（EIA）。在竞争反应中，将胃泌素抗体与胃泌素标准品（或样品）以及β-D-半乳糖苷酶标记的合成C末端胃泌素I片段（残基2 - 17）一起孵育。使用包被有抗兔IgG的免疫板分离游离的和与抗体结合的酶半抗原。通过荧光法测定板上酶的活性。目前的免疫测定法能够检测出1至20飞摩尔/毫升（2.1至42皮克/毫升）的胃泌素。测定的人血浆中G-IS水平在午餐前为7.8±1.6皮克/毫升，午餐后为26.4±8.4皮克/毫升。
