Lawler S, Candia A F, Ebner R, Shum L, Lopez A R, Moses H L, Wright C V, Derynck R
Department of Growth and Development, University of California, San Francisco 94143-0640.
Development. 1994 Jan;120(1):165-75. doi: 10.1242/dev.120.1.165.
We have isolated cDNAs of the murine type II TGF-beta receptor and have found a conserved cytoplasmic domain, but a less extensive homology in the extracellular receptor domain between the human and murine homologues. In situ hybridization analysis of the mouse fetus during mid gestation localized the expression of this receptor to various developing tissues, primarily in the mesenchyme and epidermis. This expression pattern correlates well with the expression of TGF-beta in general and especially TGF-beta 1, suggesting that TGF-beta 1 exerts its developmental role through this receptor in an autocrine or paracrine fashion. Type II receptor expression was not detected in the central nervous system and developing cartilage. These tissues lack TGF-beta 1 expression but express TGF-beta 2 and/or TGF-beta 3, suggesting that they may exert their activities through separate receptor isoforms. In addition, the efficient binding of TGF-beta 1, but not TGF-beta 2, to the cloned type II receptor strengthens the likelihood that additional type II receptor isoforms exist which display preferential binding to TGF-beta 2 and have their own defined role in development.
我们分离出了小鼠II型转化生长因子β（TGF-β）受体的cDNA，并发现了一个保守的胞质结构域，但在人和小鼠同源物的细胞外受体结构域中，同源性较低。对妊娠中期小鼠胎儿的原位杂交分析将该受体的表达定位到各种发育中的组织，主要是间充质和表皮。这种表达模式与TGF-β总体上尤其是TGF-β1的表达密切相关，表明TGF-β1以自分泌或旁分泌方式通过该受体发挥其发育作用。在中枢神经系统和发育中的软骨中未检测到II型受体表达。这些组织缺乏TGF-β1表达，但表达TGF-β2和/或TGF-β3，表明它们可能通过单独的受体异构体发挥作用。此外，TGF-β1而非TGF-β2与克隆的II型受体有效结合，这增加了存在额外的II型受体异构体的可能性，这些异构体对TGF-β2表现出优先结合，并在发育中具有各自明确的作用。
