Kojima T, Inoue M, Morikawa M, Horiguchi Y
Department of Pharmacology and Toxicology, Kanagawa Prefectural Public Health Laboratories, Japan.
Res Commun Chem Pathol Pharmacol. 1993 Dec;82(3):351-6.
Platelet aggregation was induced more strongly in male than in female 5, 12, and 45 week-old rats by both collagen and arachidonic acid. This is in agreement with our previous reports which suggested that the sex differences in platelet aggregation may be a primary characteristic of rat platelets. In plasma, plasmin-like activity was higher in male than in female rats. Plasmin alone induced aggregation, and low concentrations of plasmin synergistically enhanced collagen-induced aggregation in both male and female rats. Platelets potentiated plasmin generation by plasminogen activator at various Ca2+ concentrations in both male and female rats. Platelets from males displayed more efficient plasmin generation in 2 mM extracellular Ca2+ than those from females. If platelets were activated by abnormal causes in plasma, generated plasmin could make a greater contribution to the potentiated effect of platelet aggregation in male than in female rats. This study suggests that plasmin may be a partial cofactor in sex differences in platelet aggregation in the rat.
胶原蛋白和花生四烯酸诱导5周龄、12周龄和45周龄雄性大鼠血小板聚集的作用比雌性大鼠更强。这与我们之前的报道一致，即血小板聚集的性别差异可能是大鼠血小板的一个主要特征。在血浆中，雄性大鼠的纤溶酶样活性高于雌性大鼠。单独的纤溶酶可诱导聚集，低浓度的纤溶酶可协同增强雄性和雌性大鼠胶原蛋白诱导的聚集。在不同钙离子浓度下，雄性和雌性大鼠的血小板均可通过纤溶酶原激活剂增强纤溶酶的生成。在细胞外钙离子浓度为2 mM时，雄性大鼠的血小板比雌性大鼠的血小板产生更有效的纤溶酶。如果血小板在血浆中因异常原因被激活，生成的纤溶酶对雄性大鼠血小板聚集增强作用的贡献可能比雌性大鼠更大。本研究表明，纤溶酶可能是大鼠血小板聚集性别差异的部分辅助因子。
