Pei L, Omburo G, McGuinn W D, Petrikovics I, Dave K, Raushel F M, Wild J R, DeLoach J R, Way J L
Department of Medical Pharmacology and Toxicology, College of Medicine, Texas A&M University Health Science Center, College Station 77843-1114.
Toxicol Appl Pharmacol. 1994 Feb;124(2):296-301. doi: 10.1006/taap.1994.1035.
A new conceptual approach was employed to antagonize organophosphorus intoxication by using resealed carrier erythrocytes containing a recombinant phosphotriesterase. This enzyme has been reported to hydrolyze many organophosphorus compounds, including paraoxon, a potent cholinesterase inhibitor. Paraoxon is rapidly hydrolyzed by this enzyme to p-nitrophenol and diethylphosphate. Incorporation of phosphotriesterase within resealed murine erythrocytes was accomplished by hypotonic dialysis. The properties of this enzyme within these resealed erythrocytes were investigated. Addition of paraoxon to reaction mixtures containing these resealed erythrocytes loaded with phosphotriesterase resulted in the rapid hydrolysis of paraoxon. Hydrolysis of paraoxon did not occur when these carrier erythrocytes contained no phosphotriesterase. These in vitro studies suggest that carrier erythrocytes may be developed as an approach for the prophylactic and therapeutic antagonism of organophosphorus intoxication.
采用了一种新的概念方法，通过使用含有重组磷酸三酯酶的重封载体红细胞来对抗有机磷中毒。据报道，这种酶能水解许多有机磷化合物，包括对氧磷，一种有效的胆碱酯酶抑制剂。对氧磷能被这种酶迅速水解为对硝基苯酚和磷酸二乙酯。通过低渗透析将磷酸三酯酶掺入重封的小鼠红细胞内。研究了这种酶在这些重封红细胞内的特性。向含有装载了磷酸三酯酶的这些重封红细胞的反应混合物中加入对氧磷，会导致对氧磷迅速水解。当这些载体红细胞不含磷酸三酯酶时，对氧磷不会发生水解。这些体外研究表明，载体红细胞可能被开发成为一种预防和治疗有机磷中毒的方法。
