Bulatov A A, Osipova T A, Grigor'ian A L, Fedotov V P
Vopr Med Khim. 1994 Jan-Feb;40(1):21-5.
An immunosorbent with high binding capacity was developed using monoclonal antibodies specific to human growth hormone. Potential activity of the sorbent was studied in isolation of growth hormone from biological tissues and fluids. The immunosorbent enabled one to isolate the biologically and immunologically active growth hormone with high purity from the hypophyseal extract using only one step of purification. As shown by affinity chromatography and subsequent electrophoresis in polyacrylamide gel combined with radioimmunoblotting, together with predominant form of growth hormone, also intact and secreted from hypophysis, two-chain form of the hormone arising after proteolysis and large immunoreactive hormone fragments were detected in human circulation. These derivatives of the hormone may be also excreted with urine in unaltered form.
利用针对人生长激素的单克隆抗体研制出了一种具有高结合能力的免疫吸附剂。研究了该吸附剂从生物组织和体液中分离生长激素的潜在活性。该免疫吸附剂仅通过一步纯化就能从垂体提取物中以高纯度分离出具有生物学活性和免疫活性的生长激素。亲和色谱以及随后结合放射免疫印迹的聚丙烯酰胺凝胶电泳结果表明，除了垂体完整分泌的主要形式的生长激素外，在人体循环中还检测到了蛋白水解后产生的激素双链形式以及大的免疫反应性激素片段。这些激素衍生物也可能以未改变的形式随尿液排出。
