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用放射性气相色谱法分析挥发性醛类(作者译)

[Analysis of volatile aldehydes by radio-gaschromatography (author's transl)].

作者信息

Heimann W, Franzen K H, Rapp A, Ullemeyer H

出版信息

Z Lebensm Unters Forsch. 1975 Sep 29;159(1):1-5. doi: 10.1007/BF01089873.

Abstract

Soya- and oats-lipoxygenase (E.C. 1.13.1.13.) are incubated by 14C-marked linoleic acid. The volatile aldehydes arising thereby are isolated. The activity of the components separated by gaschromatography is written down by a printing indicator and the impulses/min are registered and printed out by a ratemeter. Thus the aldehydes which are produced by the enzymatic oxydation with lipoxygenase from the molecule of the linoleic acid can be determined. The composition of the mixture of aldehyde is calculated in mol-% from the measured impulses per peak. A possible origin of pathway is indicated for the main reaction products hexanal (soya-lipoxygenase) and non-trans-2-enal (oats-lipoxygenase).

摘要

大豆和燕麦脂氧合酶(E.C. 1.13.1.13.)与14C标记的亚油酸一起孵育。由此产生的挥发性醛被分离出来。通过气相色谱分离的各组分的活性由打印指示器记录下来,脉冲数/分钟由速率计记录并打印出来。这样就可以测定由脂氧合酶对亚油酸分子进行酶促氧化产生的醛。根据每个峰的测量脉冲,以摩尔百分比计算醛混合物的组成。指出了主要反应产物己醛(大豆脂氧合酶)和反式-2-烯醛(燕麦脂氧合酶)可能的途径来源。

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