Immunohistological localization of autoantigens detected by serum autoantibodies from mice with experimental autoimmune orchitis without using adjuvants.

Recently, a murine model of testicular autoimmunity was produced in mice by subcutaneous injections with viable syngeneic testicular germ cells alone, without depending on the use of any adjuvants or immunopotentiators. In this article, histological localization of autoantigens detected by circulating autoantibodies was immunohistochemically studied by reacting the immune sera with frozen sections of testes from mice of various ages. The study showed that the reactivity of immune sera was detected in the testis sections of normal mice older than 24 days of age but not in those of mice younger than 20 days of age. The immunostain was detected at proacrosomal, acrosomal, and other cytoplasmic regions of variously shaped developing spermatids but never on pachytene spermatocytes, basal compartment germ cells, or the supporting tissue of the seminiferous tubules.