Baracca A, Barzanti V, Lenaz G, Solaini G
Scuola Superiore di Studi Universitari e di Perfezionamento S. Anna, sezione di Medicina e Chirurgia, Pisa, Italy.
Biochem Biophys Res Commun. 1994 Feb 28;199(1):99-105. doi: 10.1006/bbrc.1994.1200.
Three groups of adult rats were fed black currant or olive oils or a 1:1 mixture of the two for three months. Feeding black currant oil diets, high in 18:2 (n-6), 18:3 (n-6), 18:3 (n-3), increased the heart and liver plasma membrane content of linoleic and arachidonic acid with a concomitant decrease of oleic acid. PUFA, n-3 and n-6 content and the bilayer lipid fluidity as examined by measuring the fluorescence anisotropy of diphenylhexatriene were not significantly affected. On the contrary, the 5'-nucleotidase of liver membrane of rats fed black currant diets was lower than that observed in membranes of liver from olive oil fed rats. Therefore it is concluded that PUFA and n-3/n-6 ratio as well as membrane fluidity do not influence the 5'-nucleotidase activity. It is suggested that the activity is sensitive to the amount of a specific fatty acid of the membrane (i.e., oleic or arachidonic acid) and/or to lipid supplementation which can influence the eicosanoids metabolism.
将三组成年大鼠分别喂食黑加仑油、橄榄油或二者1:1的混合物，为期三个月。喂食富含18:2（n-6）、18:3（n-6）、18:3（n-3）的黑加仑油饮食，会增加心脏和肝脏质膜中亚油酸和花生四烯酸的含量，同时油酸含量降低。通过测量二苯基己三烯的荧光各向异性检测的多不饱和脂肪酸（PUFA）、n-3和n-6含量以及双层脂质流动性均未受到显著影响。相反，喂食黑加仑饮食的大鼠肝细胞膜的5'-核苷酸酶低于喂食橄榄油的大鼠肝细胞膜中的5'-核苷酸酶。因此得出结论，PUFA和n-3/n-6比值以及膜流动性不会影响5'-核苷酸酶活性。有人提出，该活性对膜中特定脂肪酸（即油酸或花生四烯酸）的量和/或对可影响类花生酸代谢的脂质补充敏感。
