碳酸铜作为固相反应器用于在不分段连续流动装置中分光光度法测定多西环素和土霉素。

Copper carbonate as a solid-bed reactor for spectrophotometric determination of doxycycline and oxytetracycline in an unsegmented continuous flow assembly.

作者信息

Lopez Paz J L, Martinez Calatayud J

机构信息

Departamento de Quimica Analitica, Universitat de Valencia, Spain.

出版信息

J Pharm Biomed Anal. 1993 Nov-Dec;11(11-12):1093-8. doi: 10.1016/0731-7085(93)80087-h.

DOI:10.1016/0731-7085(93)80087-h

PMID:8123718

Abstract

The FIA-spectrophotometric determination of doxycycline was carried out by reaction of the drug with cupric ions entrapped in a polymeric material in a packed-bed reactor: the complex formed was then injected into a manifold with an alkaline solution as carrier. The developed colour was monitored at 395.0 nm. The method was applied to the determination of doxycycline in different pharmaceutical formulations. The calibration graph for doxycycline hyclate was linear over the range 10.0-80.0 mg ml-1 (n = 8) with a relative standard deviation of 1.4% (at 25 mg ml-1) and a sample throughput of 128 h-1. The proposed procedure was also applied to the determination of oxytetracycline in pharmaceutical formulations.

摘要

采用流动注射-分光光度法测定多西环素，方法是使该药物与包埋在填充床反应器中聚合材料内的铜离子反应：形成的络合物随后以碱性溶液为载体注入流路系统。在395.0 nm处监测显色情况。该方法用于测定不同药物制剂中的多西环素。盐酸多西环素的校准曲线在10.0 - 80.0 mg ml⁻¹范围内呈线性（n = 8），相对标准偏差为1.4%（在25 mg ml⁻¹时），进样通量为128 h⁻¹。所提出的方法也用于测定药物制剂中的土霉素。