Zhou Yan-Mei, Zhang Cheng-Li, Lei Jian-Du, Ma Tong-Sen, Ma Guang-Hui, Su Zhi-Guo, Chao Yang
College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1745-9.
Doxycycline hydrochloride can enhance the chemiluminescence of potassium ferricyanide and luminol in alkaline medium. So a molecular imprinting-flow-injection chemiluminescence method for the determination of doxycycline hydrochloride was established by using doxycycline hydrochloride-imprinted polymers as recognition material and potassium ferricyanide and luminol as detection system. Doxycycline hydrochloride-imprinted polymer was synthesized using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. The linear range is 9.0 x 10(-7)-6.0 x 10(-5) g x mL(-1) and the detection limit is 3.2 x 10(-7) g x mL(-1). The relative standard deviation for 6.0 X 10(-6) g x mL(-1) of doxycycline hydrochloride was 3.5% (n = 9). This method has been successfully applied to the determination of doxycycline hydrochloride in tablets and in urine samples.
盐酸多西环素在碱性介质中能增强铁氰化钾和鲁米诺的化学发光。因此，以盐酸多西环素印迹聚合物为识别材料，铁氰化钾和鲁米诺为检测体系，建立了一种测定盐酸多西环素的分子印迹-流动注射化学发光方法。以甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂合成了盐酸多西环素印迹聚合物。线性范围为9.0×10⁻⁷ - 6.0×10⁻⁵ g·mL⁻¹，检出限为3.2×10⁻⁷ g·mL⁻¹。6.0×10⁻⁶ g·mL⁻¹盐酸多西环素的相对标准偏差为3.5%（n = 9）。该方法已成功应用于片剂和尿液样品中盐酸多西环素的测定。
