采用高效薄层色谱法和扫描密度计法分析新型抗肿瘤药物FCE 23762（盐酸甲氧基吗啉多柔比星）。
Analysis of FCE 23762 (methoxymorpholinodoxorubicin hydrochloride), a new antitumour agent, by HPTLC and scanning densitometry.
作者信息
Farina A, Quaglia M G, Doldo A, Calandra S, Gallo F R
机构信息
Istituto Superiore di Sanità, Roma, Italy.
出版信息
J Pharm Biomed Anal. 1993 Nov-Dec;11(11-12):1215-8. doi: 10.1016/0731-7085(93)80106-b.
A simple, rapid and reproducible high-performance thin-layer chromatographic (HPTLC) method using UV or fluorescence scanning densitometry has been developed for the assay and purity control of methoxymorpholinodoxorubicin hydrochloride (FCE 23762). With a mobile phase of chloroform-methanol-acetic acid (93:6:1, v/v/v) and a silica gel plate, all potential impurities were separated from the main component and from each other. Detection limits at a signal-to-noise ratio of 2:1 were a few nanograms for UV detection and < 1 ng for fluorescence emission. The RSD values for the examined compounds were all < 3%.
已开发出一种简单、快速且可重现的高效薄层色谱（HPTLC）方法，采用紫外或荧光扫描密度测定法来测定盐酸甲氧基吗啉多柔比星（FCE 23762）并控制其纯度。使用氯仿 - 甲醇 - 乙酸（93:6:1，v/v/v）作为流动相和硅胶板，所有潜在杂质均与主要成分以及彼此分离。在信噪比为2:1时，紫外检测的检测限为几纳克，荧光发射的检测限小于1纳克。所检测化合物的相对标准偏差（RSD）值均小于3%。
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