用于测定生物样品中士的宁和番木鳖碱的高效薄层色谱法的评估。
Evaluation of an HPTLC method for the determination of strychnine and crimidine in biological samples.
作者信息
De Saqui-Sannes P, Nups P, Le Bars P, Burgat V
机构信息
Laboratoire associé INRA de Physiopathologie et Toxicologie expérimentales, Ecole Nationale Veterinaire de Toulouse, France.
出版信息
J Anal Toxicol. 1996 May-Jun;20(3):185-8. doi: 10.1093/jat/20.3.185.
In an attempt to improve the sensitivity and selectivity of thin-layer chromatographic analysis for the detection of strychnine and crimidine in biological samples, a rapid high-performance thin-layer chromatographic (HPTLC) method with densitometry is described. Fortified dog serum and stomach content samples were analyzed after extraction with chloroform. Quantitation was achieved by densitometry in the ultraviolet (UV) range (260 nm) of HPTLC silica gel 60 plates. Detection of trace levels as low as 5 ng proved feasible. Linearity was obtained over a range of 10-250-ng deposits for crimidine and 12.5-250-ng deposits for strychnine with simple or F254 plates. No interferences were observed in the UV spectra (220-380 nm) when peaks obtained with HPTLC of the standard substances and positive biological contents were scanned.
为提高生物样品中士的宁和环毒蝇碱检测的薄层色谱分析的灵敏度和选择性，本文描述了一种采用光密度法的快速高效薄层色谱（HPTLC）方法。用氯仿萃取后，对添加了标准物的犬血清和胃内容物样品进行分析。通过在HPTLC硅胶60板的紫外（UV）范围（260 nm）内进行光密度测定来实现定量。结果表明，检测低至5 ng的痕量水平是可行的。使用普通板或F254板时，环毒蝇碱在10 - 250 ng点样量范围内、士的宁在12.5 - 250 ng点样量范围内呈线性关系。对标准物质和阳性生物样品的HPTLC峰在220 - 380 nm紫外光谱范围内进行扫描时，未观察到干扰。
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