Lesiewicz J L, Herson D S
Arch Microbiol. 1975 Oct 27;105(2):95-9. doi: 10.1007/BF00447120.
The studies described indicate that the UV bleached mutant, Euglena gracilis W3BUL does not serve as a suitable cytoplasmic control for the phenylalanyl-tRNA synthetase system. Chromatography of wild-type E. gracilis on Sephadex G100 revealed three peaks of activity identified as the chloroplastic, cytoplasmic and mitochondrial enzymes. The chloroplastic activity was greater in log than in stationary phase cells and was the only activity recovered from purified chloroplasts. Cell-free extracts of the achloroplstic mutant, E. gracilis W3BUL, contained wild-type levels of the cytoplasmic and mitochondrial phenylalanyl-tRNA synthetases. However, no chloroplastic synthetase was detected in the mutant extracts. Anomalies in the aminoacylation behavior of the W3BUL system were observed which suggest the possibility of a mutation affecting non-chloroplastic tRNAs in this UV-induced mutant. These anomalies significantly reduce the ability of the E. gracilis W3BUL mutant to serve as a cytoplasmic control in the phenylalanyl-tRNA synthetase system.
上述研究表明，紫外线漂白突变体纤细裸藻W3BUL并非苯丙氨酰 - tRNA合成酶系统合适的细胞质对照。野生型纤细裸藻在葡聚糖G100上进行色谱分析，显示出三个活性峰，分别鉴定为叶绿体、细胞质和线粒体酶。叶绿体活性在对数期细胞中比在稳定期细胞中更高，并且是从纯化叶绿体中回收的唯一活性。无叶绿体突变体纤细裸藻W3BUL的无细胞提取物含有野生型水平的细胞质和线粒体苯丙氨酰 - tRNA合成酶。然而，在突变体提取物中未检测到叶绿体合成酶。观察到W3BUL系统氨酰化行为的异常，这表明在这个紫外线诱导的突变体中可能存在影响非叶绿体tRNA的突变。这些异常显著降低了纤细裸藻W3BUL突变体作为苯丙氨酰 - tRNA合成酶系统细胞质对照的能力。
