Morimoto Y, Yamato H, Kido M, Tanaka I, Higashi T, Fujino A, Yokosaki Y
Division of Respiratory Disease, University of Occupational and Environmental Health, Kitakyushu, Japan.
Occup Environ Med. 1994 Jan;51(1):62-7. doi: 10.1136/oem.51.1.62.
To evaluate the biological effect of ceramic fibres on the clearance function of alveolar macrophages (AMs) morphological changes and phagocytic activity of AMs were assessed. Rats were exposed to respirable ceramic fibres with a mass median aerodynamic diameter of 4.4 microns and a concentration of 20.1 mg/m3 in an exposure chamber. They were killed after one week (group A) and two weeks (group B) of exposure, and four weeks (group C) and 12 weeks (group D) after exposure for two weeks. The AMs recovered by bronchoalveolar lavage (BAL) from each test group were incubated with yeast and phagocytic activity was determined by counting the number of yeast cells in AMs. Morphological features of AMs were assessed by scanning electron microscopy and quantified according to morphological changes. Total cell counts in BAL fluid from exposed rats in group A were higher than in control rats. Phagocytic activity of exposed AMs in group B and C exceeded that of control AMs. Morphological changes of the exposed AMs in groups A, B, and C were greater than those of control AMs. These findings suggest that ceramic fibres induced the phagocytic activity and morphological changes in AMs, and that the clearance function of AMs was stimulated by the inhaled ceramic fibres.
为评估陶瓷纤维对肺泡巨噬细胞（AMs）清除功能的生物学效应，对AMs的形态变化和吞噬活性进行了评估。将大鼠置于暴露舱中，暴露于质量中位空气动力学直径为4.4微米、浓度为20.1毫克/立方米的可吸入陶瓷纤维中。在暴露1周（A组）和2周（B组）后，以及在暴露2周后的4周（C组）和12周（D组）将其处死。对每个试验组通过支气管肺泡灌洗（BAL）回收的AMs与酵母一起孵育，并通过计算AMs中酵母细胞的数量来测定吞噬活性。通过扫描电子显微镜评估AMs的形态特征，并根据形态变化进行量化。A组暴露大鼠的BAL液中的总细胞计数高于对照大鼠。B组和C组暴露的AMs的吞噬活性超过对照AMs。A组、B组和C组暴露的AMs的形态变化大于对照AMs。这些发现表明，陶瓷纤维诱导了AMs的吞噬活性和形态变化，并且吸入的陶瓷纤维刺激了AMs的清除功能。
