Islam K, Islam M Q
Department of Genetics, University of Gothenburg, Sweden.
Cytogenet Cell Genet. 1994;66(3):177-80. doi: 10.1159/000133695.
We report here the assignment of TK1, the gene for thymidine kinase to Syrian hamster (Mesocricetus auratus) chromosome 9 (MAU9) by complementation mapping. Syrian hamster chromosomes derived from a wild type (TK+) subline of BHK cells were introduced via microcell-mediated chromosome transfer into B82 mouse cells deficient in thymidine kinase (TK-), a defect that prevents their growth in HAT culture media. Hybrid clones were selected in HAT media. Chromosome analyses of the microcell hybrids showed that the thymidine kinase deficiency of B82 cells was corrected by MAU9. Therefore, we assigned TK1 to MAU9. Previously, TK1 was assigned to mouse chromosome 11 (MMU11), rat chromosome 10 (RNO10), Chinese hamster chromosome 7 (CGR7), and human chromosome 17 (HSA17). The striking banding homology of MAU9 with RNO10, MMU11, CGR7 and HSA17 provides additional support for the assignment of TK1 to MAU9. To our knowledge, this is the first report of gene assignment to a specific Syrian hamster chromosome using the somatic cell hybridization technique.
我们在此报告，通过互补定位将胸苷激酶基因TK1定位于叙利亚仓鼠（Mesocricetus auratus）的9号染色体（MAU9）上。源自BHK细胞野生型（TK+）亚系的叙利亚仓鼠染色体通过微细胞介导的染色体转移被导入缺乏胸苷激酶（TK-）的B82小鼠细胞中，这种缺陷阻止了它们在HAT培养基中的生长。在HAT培养基中筛选杂交克隆。对微细胞杂种的染色体分析表明，B82细胞的胸苷激酶缺陷被MAU9校正。因此，我们将TK1定位于MAU9。此前，TK1已被定位于小鼠11号染色体（MMU11）、大鼠10号染色体（RNO10）、中国仓鼠7号染色体（CGR7）和人类17号染色体（HSA17）。MAU9与RNO10、MMU11、CGR7和HSA17显著的带型同源性为将TK1定位于MAU9提供了额外支持。据我们所知，这是首次使用体细胞杂交技术将基因定位于特定叙利亚仓鼠染色体的报告。
