Hozier J, Scalzi J, Sawyer J, Carley N, Applegate M, Clive D, Moore M M
Department of Biological Science, Florida Institute of Technology, Melbourne.
Genomics. 1991 Jul;10(3):827-30. doi: 10.1016/0888-7543(91)90469-u.
We report the cytogenetic mapping of the thymidine kinase (tk-1) gene in the mouse using two complementary and independent analyses: (1) investigation of chromosome aberrations associated with tk-1 gene inactivation in the L5178Y TK+/- -3.7.2C cell line, and (2) fluorescence in situ molecular hybridization of cloned tk-1 cDNA probes to mitotic chromosomes of this cell line. The consensus location from both analyses is 11E1-E2. Consideration of the mouse tk-1 gene localization, along with evidence that the homologous human TK1 gene is located distally on the large arm of chromosome 17, appears to extend the region of homology between MMU11 and HSA17 to the distal end of both chromosomes.
我们通过两种互补且独立的分析方法报告了小鼠胸苷激酶（tk-1）基因的细胞遗传学定位：（1）研究L5178Y TK+/- -3.7.2C细胞系中与tk-1基因失活相关的染色体畸变，以及（2）将克隆的tk-1 cDNA探针与该细胞系的有丝分裂染色体进行荧光原位分子杂交。两种分析得出的一致定位是11E1-E2。考虑到小鼠tk-1基因的定位，以及同源的人类TK1基因位于17号染色体长臂远端的证据，似乎将MMU11和HSA17之间的同源区域扩展到了两条染色体的远端。
