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白色念珠菌原生质体融合形成的杂种分析。

Analysis of hybrids of Candida albicans formed by protoplast fusion.

作者信息

Law C, Kavanagh K, Whittaker P

机构信息

Department of Biology, Maynooth College, Kildare, Ireland.

出版信息

FEMS Microbiol Lett. 1994 Jan 1;115(1):77-82. doi: 10.1111/j.1574-6968.1994.tb06617.x.

DOI:10.1111/j.1574-6968.1994.tb06617.x
PMID:8125250
Abstract

A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained many more of the proteins associated with one parent than the other. The adherence values of the hybrids were closest to the parent with which they shared most cell wall proteins and it is suggested that the hybrids contain the genome of this parent along with one or more of the chromosomes of the other.

摘要

对通过白色念珠菌互补营养缺陷型菌株原生质体融合获得的一些杂种进行了生长速率、细胞体积、DNA含量、稳定性以及对人颊上皮细胞黏附性的分析。细胞壁蛋白的SDS-PAGE分析表明,杂种细胞壁所含与一个亲本相关的蛋白比另一个亲本多得多。杂种的黏附值最接近与其共享大多数细胞壁蛋白的亲本,并且表明杂种包含该亲本的基因组以及另一个亲本的一条或多条染色体。

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引用本文的文献

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Characterization of Candida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis.通过随机扩增多态性DNA聚合酶链反应、同工酶分析和致病性分析对白色念珠菌菌落形态突变体及其杂交体进行鉴定。
Folia Microbiol (Praha). 2004;49(5):527-33. doi: 10.1007/BF02931528.