Imbert-Bernard C, Valentin A, Mallie M, Bastide J M
Laboratoire de Parasitologie-Immunologie, Unité de Formation et de Recherche des Sciences Pharmaceutiques, Faculté de Pharmacie, Montpellier, France.
Exp Mycol. 1995 Dec;19(4):247-53. doi: 10.1006/emyc.1995.1031.
The adherence of Candida albicans to epithelial cells is one of the first steps in the development of candidiasis and therefore could constitute an interesting target for the prevention of infection. A yeast cell wall extract was prepared by using a C. albicans isolate (IVP 1453) highly adherent to buccal epithelial cells (BECs). This cell wall extract was separated by concanavalin A-affinity chromatography into two fractions referred to as Fr1 (proteic fraction) and Fr2 (mannoproteic fraction). The adhesion activity was mostly associated with the proteic fraction. This fraction was therefore retained and further fractionated by ion-exchange chromatography into two other fractions, referred to as Fr1a and Fr1b. The adhesion activity was mostly associated with the Fr1b fraction (56.4% adherence inhibition); it was not specific to the C. albicans isolate used during the cell wall extract preparation. The Fr1b fraction contained four major proteins with molecular masses of 30, 38, 47, and 54 kDa. Among these four proteins, those with molecular masses of 38 and 54 kDa could be involved in adherence mechanisms of C. albicans to human BECs.
白色念珠菌对上皮细胞的黏附是念珠菌病发展的首要步骤之一，因此可能成为预防感染的一个有意义的靶点。使用对颊上皮细胞（BECs）具有高度黏附性的白色念珠菌分离株（IVP 1453）制备酵母细胞壁提取物。该细胞壁提取物通过伴刀豆球蛋白A亲和层析分离为两个组分，分别称为Fr1（蛋白质组分）和Fr2（甘露糖蛋白组分）。黏附活性主要与蛋白质组分相关。因此保留该组分，并通过离子交换层析进一步分离为另外两个组分，称为Fr1a和Fr1b。黏附活性主要与Fr1b组分相关（黏附抑制率为56.4%）；它并非细胞壁提取物制备过程中所使用的白色念珠菌分离株所特有的。Fr1b组分包含四种主要蛋白质，分子量分别为30、38、47和54 kDa。在这四种蛋白质中，分子量为38和54 kDa的蛋白质可能参与白色念珠菌对人BECs的黏附机制。
