Sarthy A V, Schopp C, Idler K B
Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1994 Mar 11;140(1):121-6. doi: 10.1016/0378-1119(94)90741-2.
The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from Saccharomyces cerevisiae is described. The SDH1 structural gene was isolated from a lambda gt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences.
本文描述了从酿酒酵母中鉴定出一种山梨醇诱导的山梨醇脱氢酶（SDH）活性。使用针对在含山梨醇培养基中生长的酵母细胞中诱导产生的40 kDa蛋白的抗体，从λgt11酵母基因组文库中分离出SDH1结构基因。该基因编码一个由核苷酸序列推导得出的357个氨基酸（aa）的蛋白质。酵母SDH1的氨基酸序列与绵羊肝脏SDH的氨基酸序列比较显示，总体相似性为63%。含有多拷贝质粒上携带的克隆基因的酵母转化体，仅在山梨醇上生长时才高水平表达SDH1，这表明克隆基因包含调控序列和编码序列。
