Zhang J, Aronson A
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
Gene. 1994 Mar 11;140(1):85-90. doi: 10.1016/0378-1119(94)90735-8.
A 2.7-kb HindIII fragment from Bacillus subtilis contains an open reading frame (ORF) encoding a protein with homology to an Escherichia coli phospho-beta-glucosidase B (PBG B). The B. subtilis gene was induced by aromatic beta-glucosides, as judged by Northern hybridization and could complement an E. coli bglB mutant. Immediately down-stream from this B. subtilis bglA gene, there was a partial ORF on the opposite strand which encoded a polypeptide with extensive homology to NADH dehydrogenase from an alkalophilic Bacillus. These genes were mapped to 340 degrees between hut and gnt on the B. subtilis chromosome. Disruption of these genes by insertion of a neomycin-resistance-encoding gene (neo) did not result in any phenotypic changes comparable to those found in E. coli mutants.
来自枯草芽孢杆菌的一个2.7千碱基对的HindIII片段包含一个开放阅读框（ORF），该阅读框编码一种与大肠杆菌磷酸β-葡萄糖苷酶B（PBG B）具有同源性的蛋白质。通过Northern杂交判断，枯草芽孢杆菌基因由芳香族β-葡萄糖苷诱导，并且可以互补大肠杆菌bglB突变体。在这个枯草芽孢杆菌bglA基因的紧下游，在相反链上有一个部分ORF，其编码一种与嗜碱芽孢杆菌的NADH脱氢酶具有广泛同源性的多肽。这些基因被定位到枯草芽孢杆菌染色体上hut和gnt之间的340度处。通过插入新霉素抗性编码基因（neo）破坏这些基因并没有导致与在大肠杆菌突变体中发现的任何可比的表型变化。
