Xu X M, Koyama N, Cui M, Yamagishi A, Nosoh Y, Oshima T
Department of Life Science, Tokyo Institute of Technology, Kanagawa.
J Biochem. 1991 May;109(5):678-83. doi: 10.1093/oxfordjournals.jbchem.a123440.
The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (Mr 55,830). The predicted amino acid sequence was consistent with the partial amino acid sequences including the N-terminal and C-terminal sequences determined in a previous study. Sequence comparison with other flavoenzymes revealed high homology between the present dehydrogenase and Escherichia coli thioredoxin reductase.
对嗜碱芽孢杆菌属（Bacillus sp.）中编码NADH脱氢酶的基因进行了克隆和测序。克隆的DNA片段包含一个1557个核苷酸的开放阅读框，该阅读框编码一个由519个氨基酸残基组成的多肽（分子量55,830）。预测的氨基酸序列与先前研究中确定的包括N端和C端序列的部分氨基酸序列一致。与其他黄素酶的序列比较显示，目前的脱氢酶与大肠杆菌硫氧还蛋白还原酶之间具有高度同源性。
