Effros R B, Zhu X, Walford R L
Department of Pathology and Laboratory Medicine, UCLA School of Medicine.
J Gerontol. 1994 Mar;49(2):B65-70. doi: 10.1093/geronj/49.2.b65.
Senescent human T lymphocyte cultures are unable to undergo proliferation, but show no difference from early passage cells in cytotoxic function or surface antigenic profile. A second feature of senescent T cells is the dramatic reduction in hsp70 production in response to heat shock. This decline is associated with a decrease in binding of nuclear extracts to the consensus heat shock element. Interestingly, the progressive decline in the heat shock response of cultured T cells correlates with the percent proliferative life span completed rather than with the actual proliferative activity at the time of heat shock. This suggests that for senescent T cells the reduced ability to respond to heat shock by producing hsp70, although possibly lying at the level of transcriptional control, may nevertheless be unrelated to the reduced DNA synthesis or the diminished proliferative activity also manifested by these cells.
衰老的人类T淋巴细胞培养物无法进行增殖，但在细胞毒性功能或表面抗原谱方面与早期传代细胞没有差异。衰老T细胞的第二个特征是热休克后hsp70产生显著减少。这种下降与核提取物与共有热休克元件的结合减少有关。有趣的是，培养的T细胞热休克反应的逐渐下降与完成的增殖寿命百分比相关，而不是与热休克时的实际增殖活性相关。这表明，对于衰老的T细胞来说，通过产生hsp70对热休克作出反应的能力降低，尽管可能处于转录控制水平，但仍可能与这些细胞中DNA合成减少或增殖活性降低无关。
