López de Felipe F
Servicio de Bacteriología, Centro Nacional de Microbiología, Madrid, Spain.
J Gen Microbiol. 1993 Dec;139(12):3171-5. doi: 10.1099/00221287-139-12-3171.
A plasmid designated pLPG36 was isolated from the naturally occurring Legionella pneumophila serogroup-1 and purified by CsCl buoyant density centrifugation. A restriction map of this 58 kb plasmid was constructed and provided the basis for cloning four BamHI fragments into the unique BamHI site of pUC18. The four recombinant plasmids were investigated for the mobilization function in Escherichia coli strains. Only one of these, pFLJ2, was mobilized by the IncP plasmids RP4, pRK231 and R702, but not by plasmids pSa, R40a, R387, pN3 or R16. The derivative plasmid pFLJ2 was mobilized more efficiently by R702 than by RP4 or pRK231. By genetic and deletion analysis, the mobilization region of pLPG36 was located to a 6 kb EcoRI fragment of the plasmid.
从天然存在的嗜肺军团菌血清型1中分离出一种名为pLPG36的质粒，并通过氯化铯浮力密度离心法进行纯化。构建了这个58 kb质粒的限制性图谱，为将四个BamHI片段克隆到pUC18的独特BamHI位点提供了基础。研究了这四个重组质粒在大肠杆菌菌株中的转移功能。其中只有一个，即pFLJ2，可被IncP质粒RP4、pRK231和R702转移，但不能被质粒pSa、R40a、R387、pN3或R16转移。衍生质粒pFLJ2被R702转移的效率高于被RP4或pRK231转移的效率。通过基因和缺失分析，pLPG36的转移区域定位于该质粒的一个6 kb EcoRI片段。
