dutA RNA在盘基网柄菌的发育过程中作为一种不可翻译的RNA发挥作用。

dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum.

作者信息

Yoshida H, Kumimoto H, Okamoto K

机构信息

Department of Botany, Faculty of Science, Kyoto University, Japan.

出版信息

Nucleic Acids Res. 1994 Jan 11;22(1):41-6. doi: 10.1093/nar/22.1.41.

dutA is a gene specifically expressed during the development of Dictyostelium discoideum. Toward understanding its possible role in development, we isolated and characterized the gene and its complete cDNA. We found that dutA is encoded by the nuclear genome as a single copy gene without introns. In addition, the following unique and interesting features of dutA RNA (1322 nt) emerged: (1) it has no sustained ORFs (MAX = 126 nt) (2) it is extremely AU-rich (83%) (3) it contains peculiar sequence motifs (large palindromes, long AU-stretches and GC-clusters) (4) it is localized in the cytoplasm but completely absent from ribosomes. These features suggest that dutA RNA functions without being translated into protein. Disruption of the dutA gene did not cause phenotypic changes, suggesting that the function of dutA is redundant.

dutA是一种在盘基网柄菌发育过程中特异性表达的基因。为了了解其在发育过程中可能发挥的作用，我们分离并鉴定了该基因及其完整的cDNA。我们发现dutA由核基因组编码，是一个无内含子的单拷贝基因。此外，dutA RNA（1322个核苷酸）呈现出以下独特且有趣的特征：（1）它没有持续的开放阅读框（最长为126个核苷酸）；（2）它富含AU（83%）；（3）它包含特殊的序列基序（大回文序列、长AU序列和GC簇）；（4）它定位于细胞质中，但核糖体中完全没有。这些特征表明dutA RNA在不翻译成蛋白质的情况下发挥功能。dutA基因的破坏并未导致表型变化，这表明dutA的功能是冗余的。