Yun Z B, Sönnerborg A, Weiland O
Dept. of Virology, Central Microbiological Laboratory of Stockholm County Council, Sweden.
Scand J Gastroenterol. 1994 Jan;29(1):82-6. doi: 10.3109/00365529409090442.
Serum, liver tissue, and peripheral blood mononuclear cells (PBMC) were obtained from 72 patients with chronic hepatitis C, 19 of whom had received interferon-alpha (IFN-alpha) treatment. Hepatitis C virus (HCV) RNA was detected by polymerase chain reaction in the serum and liver of all patients with relapse after treatment and in 51 of 53 (96%) sera, 28 of 29 (97%) livers, and all of 13 (100%) PBMC from untreated patients but not in 3 patients with completely sustained responses. Minus-stranded HCV RNA was found in 5 of 14 (35%) sera, 16 of 17 (94%) livers, and 7 of 13 (53%) PBMC from untreated patients. The results indicate that HCV RNA is present not only in serum and liver but also in PBMC from the vast majority of untreated anti-HCV-seropositive patients and also in patients who respond to IFN-alpha treatment but then relapse. HCV replicates in the liver of almost all patients, but replication may possibly also occur in PBMC. By contrast, HCV RNA seems to be eradicated in patients with completely sustained responses.
从72例慢性丙型肝炎患者中获取血清、肝组织和外周血单个核细胞（PBMC），其中19例曾接受α干扰素（IFN-α）治疗。通过聚合酶链反应在所有治疗后复发患者的血清和肝脏中检测到丙型肝炎病毒（HCV）RNA，在未经治疗患者的53份血清中的51份（96%）、29份肝脏中的28份（97%）以及13份PBMC中的所有样本（100%）中检测到HCV RNA，但在3例完全持续应答的患者中未检测到。在未经治疗患者的14份血清中的5份（35%）、17份肝脏中的16份（94%）以及13份PBMC中的7份（53%）中发现了负链HCV RNA。结果表明，HCV RNA不仅存在于血清和肝脏中，还存在于绝大多数未经治疗的抗-HCV血清阳性患者的PBMC中，以及对IFN-α治疗有反应但随后复发的患者中。几乎所有患者的肝脏中都有HCV复制，但PBMC中也可能发生复制。相比之下，在完全持续应答的患者中HCV RNA似乎被清除。
