Okuda M
First Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan.
Gastroenterol Jpn. 1993 Aug;28(4):535-40. doi: 10.1007/BF02776952.
Hepatitis C virus RNA (HCV-RNA) was serially assayed in the serum and peripheral blood mononuclear cells (PBMC) of 10 patients with chronic hepatitis C who underwent interferon (IFN) therapy, and whether detection of HCV-RNA from PBMC serves as an index of the response of chronic hepatitis C to IFN therapy was evaluated. HCV-RNA was assayed by reversed transcription and polymerase chain reaction using the 5'-noncoding region as a primer. IFN therapy was effective in 3 patients and ineffective in the other 7 patients. HCV-RNA disappeared from the serum during and immediately after the IFN therapy in all 3 patients in whom the therapy was effective and in 3 of 7 patients in whom the therapy was ineffective. HCV-RNA disappeared from PBMC in all 3 patients in whom the therapy was effective, but PBMC HCV-RNA remained positive in 6 of the 7 patients in whom the therapy was ineffective, and the serum HCV-RNA became positive again in 5 of these 6 patients after 6 months. The disappearance of HCV-RNA from PBMC was associated with long-term stabilization of the serum alanine aminotransferase value, so HCV-RNA assay in PBMC is considered to be useful as a prognostic marker of chronic hepatitis C after IFN therapy.
对10例接受干扰素（IFN）治疗的慢性丙型肝炎患者的血清和外周血单个核细胞（PBMC）进行了丙型肝炎病毒RNA（HCV-RNA）的连续检测，并评估了从PBMC中检测HCV-RNA是否可作为慢性丙型肝炎对IFN治疗反应的指标。使用5'-非编码区作为引物，通过逆转录和聚合酶链反应检测HCV-RNA。IFN治疗对3例患者有效，对其他7例患者无效。在所有3例治疗有效的患者以及7例治疗无效患者中的3例中，IFN治疗期间及治疗后即刻血清中的HCV-RNA消失。在所有3例治疗有效的患者中，PBMC中的HCV-RNA消失，但在7例治疗无效的患者中，有6例患者的PBMC HCV-RNA仍为阳性，且在6个月后，这6例患者中有5例血清HCV-RNA再次转为阳性。PBMC中HCV-RNA的消失与血清丙氨酸氨基转移酶值的长期稳定相关，因此PBMC中的HCV-RNA检测被认为可作为IFN治疗后慢性丙型肝炎预后标志物。
