Wu H Y, Tuomikoski T, Eray M, Mattila P, Knuutila S, Kaartinen M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Scand J Immunol. 1994 Mar;39(3):295-300. doi: 10.1111/j.1365-3083.1994.tb03374.x.
Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91% homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes.
对两个新建立的人类淋巴瘤细胞系（HF - 1和HF - 4）的可变免疫球蛋白重链区域（VDJ）进行了测序。在HF - 1和HF - 4序列中发现的与种系VH基因最同源的是VH3a（V基因）家族的VH26（同源性分别为82%和91%）。与已发表的种系JH3基因相比，HF - 4重链序列的JH区域有两个核苷酸差异。HF - 1基因的DHJH区域有创纪录的高体细胞突变率（20%）。在HF - 1细胞系中发现的大量超突变支持了这样一种假说，即在一些人类滤泡性淋巴瘤中，免疫球蛋白基因在恶性生长过程中持续积累突变。具有活跃突变机制的滤泡性淋巴瘤细胞系未来可能有助于解决修饰B淋巴细胞免疫球蛋白基因的体细胞超突变背后的分子事件。
