Lampisuo M, Lassila O
Department of Medical Microbiology, Turku University, Finland.
Scand J Immunol. 1994 Mar;39(3):321-6. doi: 10.1111/j.1365-3083.1994.tb03378.x.
In the avian embryo the haemopoietic stem cells originate from the intra-embryonic area near dorsal aorta. The surface-marker expression of haemopoietic stem cells and their potential to produce different haemopoietic cells are still largely unknown. The surface antigen expression and particularly the MHC antigen expression on intra-embryonic haemopoietic cells was studied. Expression of B-F antigens, homologous to mammalian MHC class-I antigens, was found already on embryonic day (ED) 5. The first B-L antigens, analogous to mammalian MHC class-II antigens, were detected also from ED5 onwards. The appearance of surface antigens defined by MoAbs T10A6 and 3-298 during embryogenesis also was studied. The antigen defined with T10A6 was detected from ED4 onwards on endothelial cells but not on haemopoietic cells in the para-aortic region. The first 3-298+ haemopoietic cells were found on ED6, whereas endothelial cells were negative. These findings imply that some surface markers are shared with haemopoietic and endothelial cells indicating either a common embryonic origin or the importance of these molecules in embryonic stem-cell homing.
在禽类胚胎中，造血干细胞起源于背主动脉附近的胚内区域。造血干细胞的表面标志物表达及其产生不同造血细胞的潜力在很大程度上仍然未知。研究了胚内造血细胞的表面抗原表达，尤其是MHC抗原表达。在胚胎第5天就已发现与哺乳动物MHC I类抗原同源的B-F抗原的表达。同样从胚胎第5天开始也检测到了与哺乳动物MHC II类抗原类似的首批B-L抗原。还研究了单克隆抗体T10A6和3-298所定义的表面抗原在胚胎发生过程中的出现情况。用T10A6定义的抗原从胚胎第4天起在内皮细胞上被检测到，但在主动脉旁区域的造血细胞上未检测到。在胚胎第6天发现了首批3-298+造血细胞，而此时内皮细胞呈阴性。这些发现表明，一些表面标志物在造血细胞和内皮细胞中是共有的，这表明它们要么有共同的胚胎起源，要么这些分子在胚胎干细胞归巢中具有重要性。
