Gogusev J, Suskind G, Mongiat F, Hlozánek I
Folia Biol (Praha). 1987;33(2):104-13.
The expression and distribution of chicken major histocompatibility antigens (H-B) was examined by electron microscopy. Using an indirect method of ferritin and peroxidase labelling with monospecific heteroantisera and with monoclonal antibodies, the H-B alloantigens could be localized at the plasma membrane of various cell types. The antigenic binding sites were quantified on mature erythrocytes, erythropoietic cells, heterophils, lymphoid cells of both thymus and bursa origin, and on cultured normal and Rous sarcoma virus-transformed chick embryo fibroblasts. The degree of labelling was higher in mature normoblastic erythrocytes and lymphoid cells than in other haemopoietic cells and fibroblasts when the heterospecific antisera were used. A larger number of antigenic binding sites was labelled by the monomeric monoclonal antibodies than by the polyclonal antisera. The distribution and aggregation of the specific label on the plasma membrane of cells other than erythrocytes, however, differed in the fixed and unfixed cells.
采用电子显微镜检查鸡主要组织相容性抗原（H - B）的表达和分布情况。运用铁蛋白和过氧化物酶间接标记法，结合单特异性异种抗血清及单克隆抗体，可将H - B同种异体抗原定位在多种细胞类型的质膜上。对成熟红细胞、造血细胞、嗜异性粒细胞、胸腺和法氏囊来源的淋巴细胞，以及培养的正常和劳氏肉瘤病毒转化的鸡胚成纤维细胞上的抗原结合位点进行了定量分析。当使用异种特异性抗血清时，成熟的正成红细胞和淋巴细胞中的标记程度高于其他造血细胞和成纤维细胞。与多克隆抗血清相比，单体单克隆抗体标记的抗原结合位点数量更多。然而，除红细胞外的其他细胞质膜上特异性标记的分布和聚集情况，在固定细胞和未固定细胞中有所不同。
