Tsopanakis C, Parish J H
J Gen Virol. 1976 Jan;30(1):99-112. doi: 10.1099/0022-1317-30-1-99.
Bacteriophage MX-1 is a virulent DNA phage for Myxococcus. The host range includes strains of Myxococcus xanthus, M. fulvus and M. virescens. The phage has a sedimentation coefficient (S degrees 20,w) of 1145S and a density of 1-531 g/ml. By using SDS-polyacrylamide gel electrophoresis, 23 phage proteins with apparent mol. wt. between 10000 and 150000 were resolved. Gel filtration in the presence of non-ionic detergent partially resolved the proteins. The fraction excluded from Sephadex G-100, fraction 1, contains two glycoproteins. Fraction 1 was resolved into three fractions (1-1, 1-2 and 1-3) by chromatography on Sephadex G-200. The glycoproteins were present in fraction 1-2; all the proteins from this fraction were derived from the phage tail. Comparison of the amino-acid, hexosamine and neutral-sugar compositions of the two glycoproteins showed that they are distinct molecular species; the smaller molecule is not a subunit of the larger. The significance of these findings is discussed and compared with the proteins of the tails of T-even phage of Escherichia coli.
噬菌体MX-1是一种针对黏球菌的烈性DNA噬菌体。宿主范围包括黄色黏球菌、褐黄黏球菌和绿色黏球菌的菌株。该噬菌体的沉降系数（S°20,w）为1145S，密度为1.531 g/ml。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，分辨出了23种表观分子量在10000至150000之间的噬菌体蛋白。在非离子去污剂存在下进行凝胶过滤可部分分离这些蛋白。从葡聚糖凝胶G-100中排除的组分，即组分1，包含两种糖蛋白。通过在葡聚糖凝胶G-200上进行色谱分析，组分1被分离为三个组分（1-1、1-2和1-3）。糖蛋白存在于组分1-2中；该组分中的所有蛋白均来自噬菌体尾部。对这两种糖蛋白的氨基酸、己糖胺和中性糖组成进行比较表明，它们是不同的分子种类；较小的分子不是较大分子的亚基。本文讨论了这些发现的意义，并与大肠杆菌T偶数噬菌体尾部的蛋白进行了比较。
