Stellwag E, Fink J M, Zissler J
Mol Gen Genet. 1985;199(1):123-32. doi: 10.1007/BF00327521.
We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7 +/- 0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome.
我们使用PvuII、MboI和EcoRI酶构建了来自黄色粘球菌的噬菌体MX-8基因组的限制酶切图谱。通过限制酶切分析确定，噬菌体基因组大小为51.7±0.6 kb。双酶切、分离片段的再酶切以及部分酶切产物的交叉接触杂交表明，该限制酶切图谱是环状的。限制酶切分析和Southern杂交表明，噬菌体DNA分子从我们已定位的一个特定位点开始，从串联体依次包装。DNA分子的平均末端冗余约为8%，并且在至少40%的基因组上呈环状排列。
