在哺乳动物体外和体内系统中鉴定眼内透镜吸附蛋白。

Identification of intraocular lens-adsorbed proteins in mammalian in vitro and in vivo systems.

作者信息

Kochounian H H, Kovacs S A, Sy J, Grubbs D E, Maxwell W A

机构信息

Department of Biology, California State University, Fresno.

出版信息

Arch Ophthalmol. 1994 Mar;112(3):395-401. doi: 10.1001/archopht.1994.01090150125034.

DOI:10.1001/archopht.1994.01090150125034

PMID:8129667

Abstract

OBJECTIVE

Mammalian in vitro and in vivo systems were used to study the protein-adsorbing potential of intraocular lenses (IOLs).

METHODS

Intraocular lenses composed of polymethyl methacrylate optics with polypropylene haptics were incubated in rabbit plasma for 3 hours (in vitro grouping) or implanted in rabbit eyes for 48 hours (in vivo grouping). Lens-adsorbed proteins from both experimental groupings were eluted with sodium dodecyl sulfate and identified by Western Blot analyses.

RESULTS

The adsorbed protein layer was composed of at least six different proteins: albumin, complement C3 fragments, IgG, fibrinogen/fibrin (as a fibrin clot in vivo), fibronectin, and transferrin. Of the identified components, albumin, IgG, fibronectin, and fibrinogen were the predominant protein species on the in vitro IOLs, while fibronectin and fibrin were on the in vivo IOLs.

CONCLUSIONS

The composition of the protein layer has the potential to alter the biological property of IOLs.

摘要

目的

利用哺乳动物的体外和体内系统研究人工晶状体（IOL）的蛋白质吸附潜力。

方法

将由聚甲基丙烯酸甲酯光学部和聚丙烯襻组成的人工晶状体在兔血浆中孵育3小时（体外组）或植入兔眼48小时（体内组）。用十二烷基硫酸钠洗脱两个实验组晶状体吸附的蛋白质，并通过蛋白质印迹分析进行鉴定。

结果

吸附的蛋白质层由至少六种不同的蛋白质组成：白蛋白、补体C3片段、免疫球蛋白G（IgG）、纤维蛋白原/纤维蛋白（在体内为纤维蛋白凝块）、纤连蛋白和转铁蛋白。在已鉴定的成分中，白蛋白、IgG、纤连蛋白和纤维蛋白原是体外人工晶状体上的主要蛋白质种类，而纤连蛋白和纤维蛋白是体内人工晶状体上的主要蛋白质种类。