Fauler J, Thon A, Tsikas D, von der Hardt H, Frölich J C
Department of Clinical Pharmacology, Hannover Medical School, Germany.
Arthritis Rheum. 1994 Jan;37(1):93-7. doi: 10.1002/art.1780370114.
Endogenous synthesis of cysteinyl leukotrienes in juvenile rheumatoid arthritis (JRA) was investigated.
Cysteinyl leukotriene synthesis was assessed by measuring the excretion of leukotriene E4 (LTE4) in urine by radioimmunoassay. The identity of urinary LTE4 was investigated by gas chromatography-mass spectrometry (GC-MS), and 2,3-dinor-thromboxane B2 was measured with GC-MS.
Excretion of LTE4 into urine was significantly (P < 0.05) enhanced in children with JRA compared with that in healthy children (n = 10). Aspirin, in a dosage of 2.5 gm/day, had no effect on urinary LTE4 levels, but it reduced urinary 2,3-dinorthromboxane B2 levels by more than 85% in healthy adults. There was a positive correlation between LTE4 excretion and the number of affected joints.
This study demonstrates a markedly enhanced cysteinyl leukotriene synthesis and a positive correlation between LTE4 excretion and the number of affected joints in children with JRA.
研究青少年类风湿性关节炎（JRA）中半胱氨酰白三烯的内源性合成。
通过放射免疫分析法测量尿中白三烯E4（LTE4）的排泄量来评估半胱氨酰白三烯的合成。通过气相色谱 - 质谱联用（GC-MS）研究尿中LTE4的特性，并用GC-MS测量2,3-二去甲血栓素B2。
与健康儿童（n = 10）相比，JRA患儿尿中LTE4的排泄量显著增加（P < 0.05）。每天2.5克剂量的阿司匹林对尿中LTE4水平没有影响，但它使健康成年人尿中2,3-二去甲血栓素B2水平降低了85%以上。LTE4排泄量与受累关节数量之间存在正相关。
本研究表明JRA患儿中半胱氨酰白三烯合成明显增强，且LTE4排泄量与受累关节数量之间存在正相关。
