Mohn G, Koehl P, Budzikiewicz H, Lefèvre J F
Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France.
Biochemistry. 1994 Mar 15;33(10):2843-51. doi: 10.1021/bi00176a014.
The three-dimensional structure in solution of ferri-pyoverdin GM-II isolated from the culture medium of Pseudomonas fluorescens was determined by application of NMR methods to the Ga3+ analogue. Distance geometry calculations were performed with FILMAN using interproton distances and coupling constants as constraints. Further conformational analysis was carried out by energy minimization with MM2 and AMBER. Back-calculation of the NOESY spectra shows that the resulting structures are in agreement with the experimental data.
通过对镓离子（Ga3+）类似物应用核磁共振（NMR）方法，确定了从荧光假单胞菌培养基中分离出的铁黄绿菌素GM-II在溶液中的三维结构。使用质子间距离和耦合常数作为约束条件，通过FILMAN进行距离几何计算。通过MM2和AMBER进行能量最小化，进一步进行构象分析。对NOESY谱的反计算表明，所得结构与实验数据一致。
