Matsui N M, Patek P Q
Department of Microbiology, University of Hawaii at Manoa, Honolulu 96822.
Cell Immunol. 1994 Apr 1;154(1):358-68. doi: 10.1006/cimm.1994.1083.
Natural cytotoxic (NC) activity is mediated by a cell-associated form of TNF-alpha. Here, we have characterized the NC-like properties of cloned murine transformed B cell lines. Several B cell lines cause the lysis of NC/TNF-alpha-susceptible target cells, but not NC/TNF-apha-resistant target cells. Furthermore, lysis of targets by B cell lines involves a nonsoluble (i.e., cell-associated) mechanism that is blocked by anti-TNF antibody. Target cells selected for resistance to a B lymphoma cell line expressing NC-like activity are resistant to splenic NC-mediated lysis, resistant to TNF-alpha-mediated lysis, and have increased tumorigenicity in BALB/c mice. Thus, these B cells lines may serve as cloned effectors which lyse cells using a cell-associated TNF-dependent mechanism analogous to NC lysis. Furthermore, it is possible that the expression of TNF by transformed B cells is autostimulatory and is involved in their malignant transformation.
自然细胞毒性（NC）活性由细胞相关形式的肿瘤坏死因子-α（TNF-α）介导。在此，我们对克隆的小鼠转化B细胞系的类NC特性进行了表征。几种B细胞系可导致对NC/TNF-α敏感的靶细胞裂解，但不会导致对NC/TNF-α抗性靶细胞的裂解。此外，B细胞系对靶细胞的裂解涉及一种不溶性（即细胞相关）机制，该机制可被抗TNF抗体阻断。选择对表达类NC活性的B淋巴瘤细胞系具有抗性的靶细胞，对脾脏NC介导的裂解具有抗性，对TNF-α介导的裂解具有抗性，并且在BALB/c小鼠中具有增强的致瘤性。因此，这些B细胞系可作为克隆效应细胞，其使用类似于NC裂解的细胞相关TNF依赖性机制裂解细胞。此外，转化B细胞中TNF的表达可能具有自刺激作用，并参与其恶性转化。
