Lattime E C, Stutman O
Division of Medical Oncology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Cell Immunol. 1991 Aug;136(1):69-79. doi: 10.1016/0008-8749(91)90382-l.
Tumor necrosis factor (TNF), lyses a range of sensitive tumor targets and has been shown to be the mediator of natural cytotoxic (NC) activity first described in our laboratory. In this report, we identify two thymic lymphoma cell lines which lyse the prototype NC target WEHI-164 and share characteristics of NC. R1.1E and L5178-27av lyse the WEHI-164 sarcoma in 18-hr 51Cr release assays via a TNF-dependent, non-MHC-restricted (R1.1E) mechanism although they do not constitutively produce TNF. NC- and TNF-resistant variants of WEHI-164 are resistant to lymphoma-mediated lysis. Expression of the ganglioside GD3 by the lymphomas correlates with their relative levels of lysis. Thus, GD3, which is known to have a role in T cell activation may be involved in recognition or triggering for TNF-dependent cell-mediated lysis.
肿瘤坏死因子（TNF）可裂解一系列敏感肿瘤靶标，并且已被证明是我们实验室首次描述的天然细胞毒性（NC）活性的介质。在本报告中，我们鉴定出两种胸腺淋巴瘤细胞系，它们可裂解典型的NC靶标WEHI-164并具有NC的特征。R1.1E和L5178-27av在18小时的51Cr释放试验中通过TNF依赖性、非MHC限制性（R1.1E）机制裂解WEHI-164肉瘤，尽管它们不能组成性地产生TNF。WEHI-164的NC抗性和TNF抗性变体对淋巴瘤介导的裂解具有抗性。淋巴瘤中神经节苷脂GD3的表达与其相对裂解水平相关。因此，已知在T细胞活化中起作用的GD3可能参与TNF依赖性细胞介导的裂解的识别或触发。
