Butcher W G, Close J, Krajewska-Pietrasik D, Switalski L M
Department of Periodontics, School of Dental Medicine, University of Pittsburgh, Pa 15261.
Chemotherapy. 1994 Mar-Apr;40(2):114-23. doi: 10.1159/000239182.
The effects of sub-minimum inhibitory concentrations (sub-MICs) of three antibiotics affecting the biosynthesis of peptidoglycan on the interactions of Staphylococcus aureus strains with collagenous substrata were evaluated. In a system measuring binding of 125I-labeled collagen, growth of bacteria in the presence of one-quarter MIC of cloxacillin and vancomycin reduced the number of collagen binding sites on the surface of bacteria. Growth in the presence of cefpodoxime reduced the number of collagen binding sites in one strain and increased it in another. Cefpodoxime also increased the dissociation constant of collagen binding to bacteria, 2- to 3-fold, while the other two antibiotics did not affect the affinity of the interaction. In a system measuring adhesion of 125I-labeled bacteria to collagen-coated surfaces or cartilage, bacteria grown in the presence of cloxacillin and vancomycin attached to varying degrees depending on the strain. In contrast, compared to untreated controls as well as to bacteria treated with the other two antibiotics, growth in the presence of cefpodoxime significantly reduced adhesion of the majority of strains tested. Sub-MICs of antibiotics appear to affect staphylococcal adhesion to collagenous substrata with cefpodoxime exhibiting the strongest effect. The critical factor in reducing bacterial adhesion seems not to be the number of bacterial binding sites for collagen, but the affinity of the interaction.
评估了三种影响肽聚糖生物合成的抗生素亚最小抑菌浓度（sub-MICs）对金黄色葡萄球菌菌株与胶原质底物相互作用的影响。在一个测量125I标记胶原结合的系统中，在氯唑西林和万古霉素四分之一MIC存在下细菌的生长减少了细菌表面胶原结合位点的数量。在头孢泊肟存在下生长，一个菌株的胶原结合位点数量减少，而在另一个菌株中则增加。头孢泊肟还使胶原与细菌结合的解离常数增加了2至3倍，而其他两种抗生素不影响这种相互作用的亲和力。在一个测量125I标记细菌对胶原包被表面或软骨粘附的系统中，在氯唑西林和万古霉素存在下生长的细菌根据菌株不同有不同程度的附着。相比之下，与未处理的对照以及用其他两种抗生素处理的细菌相比，在头孢泊肟存在下生长显著降低了大多数测试菌株的粘附。抗生素的亚最小抑菌浓度似乎影响葡萄球菌对胶原质底物的粘附，其中头孢泊肟的影响最强。降低细菌粘附的关键因素似乎不是细菌对胶原的结合位点数量，而是相互作用的亲和力。
