Voorn G P, Thompson J, Goessens W H, Schmal-Bauer W C, Broeders P H, Michel M F
Department of Infectious Diseases, University Hospital Leiden, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):741-6. doi: 10.1007/BF02276057.
The stability of tolerance of Staphylococcus aureus during subculturing at 37 degrees C and development of this property after repeated exposure to cloxacillin or vancomycin were investigated in vitro. Four of five tolerant strains lost this property during repeated subculturing at 37 degrees C for 50 days. Conversely, tolerance emerged in two of four nontolerant strains after repeated cycles of exposure to 25 micrograms of cloxacillin/ml or 10 micrograms of vancomycin/ml alternating with growth in antibiotic-free medium. Previous in vivo exposure to cloxacillin did not enhance the development of tolerance in vitro. MICs of both cloxacillin and vancomycin did not change significantly during this procedure. Whether the conversion of nontolerant strains to the tolerant state can also occur during antibiotic exposure in treatment of patients remains to be determined.
在体外研究了金黄色葡萄球菌在37℃传代培养期间耐受性的稳定性以及在反复接触氯唑西林或万古霉素后该特性的发展情况。五株耐受菌株中有四株在37℃反复传代培养50天期间失去了该特性。相反，四株非耐受菌株中有两株在反复暴露于25微克/毫升氯唑西林或10微克/毫升万古霉素并与在无抗生素培养基中生长交替进行的循环后出现了耐受性。先前在体内接触氯唑西林并未增强体外耐受性的发展。在此过程中，氯唑西林和万古霉素的最低抑菌浓度均未发生显著变化。在患者治疗过程中抗生素暴露期间非耐受菌株是否也会转变为耐受状态仍有待确定。
