Hattingh J
Pflugers Arch. 1975 Apr 2;355(4):347-52. doi: 10.1007/BF00579855.
This study compares the effects of ethylenediamine tetra-acetate (EDTA) and heparin in various concentrations as anticoagulants on the microhaematocrit values of the blood of five species of freshwater fish. EDTA always produced haemolysis after a time interval ranging from 1 to 48 hrs and increased cell volume. Heparin in powdered form was found to be the anticoagulant of choice although minor haematological changes do occur, but it is stressed that preliminary experiments must be done to examine the effects of the anticoagulant before routine haematologic analyses are preformed.
本研究比较了不同浓度的乙二胺四乙酸（EDTA）和肝素作为抗凝剂对五种淡水鱼血液微量血细胞比容值的影响。EDTA在1至48小时的时间间隔后总会导致溶血，并增加细胞体积。发现粉末状肝素是首选的抗凝剂，尽管确实会发生轻微的血液学变化，但需要强调的是，在进行常规血液学分析之前，必须进行初步实验以检查抗凝剂的效果。
