China B, N'Guyen B T, de Bruyere M, Cornelis G R
Microbial Pathogenesis Unit, Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium.
Infect Immun. 1994 Apr;62(4):1275-81. doi: 10.1128/iai.62.4.1275-1281.1994.
Pathogenic Yersinia enterocolitica cells do not induce the chemiluminescence response of human polymorphonuclear leukocytes (PMNs). We tested the chemiluminescence response to Y. enterocolitica mutants affected in the known pYV-encoded factors. We did not detect any influence of the Yops in this phenomenon. By contrast, the presence of YadA correlated with a lack of chemiluminescence. The expression of YadA at the bacterial surface also reduced the phagocytosis by PMNs. Finally, we measured the survival of Y. enterocolitica cells confronted with PMNs by the classical plating method and by a new luminometry assay. We observed that YadA+ bacteria were not killed, while YadA- bacteria were killed. We conclude that the presence of YadA at the surface of Y. enterocolitica cells prevents phagocytosis and killing by PMNs. This conclusion is in good agreement with our recent observation that YadA protects Y. enterocolitica from opsonization by C3b.
致病性小肠结肠炎耶尔森菌细胞不会诱导人多形核白细胞（PMN）的化学发光反应。我们测试了对已知pYV编码因子有缺陷的小肠结肠炎耶尔森菌突变体的化学发光反应。我们未检测到Yops在这一现象中的任何影响。相比之下，YadA的存在与化学发光的缺乏相关。YadA在细菌表面的表达也降低了PMN的吞噬作用。最后，我们通过经典平板计数法和一种新的发光测定法测量了与PMN接触的小肠结肠炎耶尔森菌细胞的存活率。我们观察到YadA +细菌未被杀死，而YadA -细菌被杀死。我们得出结论，小肠结肠炎耶尔森菌细胞表面YadA的存在可防止被PMN吞噬和杀伤。这一结论与我们最近观察到的YadA保护小肠结肠炎耶尔森菌免受C3b调理作用的结果高度一致。
