Lian C J, Pai C H
Infect Immun. 1985 Jul;49(1):145-51. doi: 10.1128/iai.49.1.145-151.1985.
Recent studies have shown that the cell surface properties of Yersinia enterocolitica are altered by the presence of the virulence plasmid, which mediates temperature-inducible outer membrane proteins (OMP). We investigated the interaction of Y. enterocolitica with human polymorphonuclear leukocytes by monitoring luminol-enhanced chemiluminescence (CL) responses. A plasmid-bearing strain grown at 37 degrees C induced four- to sixfold less CL than did the same strain grown at 25 degrees C or a plasmidless, isogenic strain grown at either temperature. Inhibition of CL responses by whole cells was related to plasmid-mediated expression of OMP. The OMP alone could inhibit the CL response of polymorphonuclear leukocytes stimulated by either opsonized zymosan or whole cells of Y. enterocolitica. Pronase treatment of whole cells, which removed the plasmid-mediated OMP, resulted in partial but significant elimination of CL inhibition by whole cells and by OMP derived from them. Incubation with Y. enterocolitica for 60 min did not affect the viability of polymorphonuclear leukocytes. Our results suggest that the interaction of Y. enterocolitica with human polymorphonuclear leukocytes is directly affected by the plasmid-mediated OMP.
最近的研究表明，毒力质粒的存在会改变小肠结肠炎耶尔森菌的细胞表面特性，该质粒介导温度诱导型外膜蛋白（OMP）。我们通过监测鲁米诺增强的化学发光（CL）反应，研究了小肠结肠炎耶尔森菌与人类多形核白细胞的相互作用。在37℃培养的携带质粒的菌株诱导的CL比在25℃培养的同一菌株或在任一温度下培养的无质粒同基因菌株诱导的CL少四至六倍。全细胞对CL反应的抑制与质粒介导的OMP表达有关。单独的OMP可以抑制经调理的酵母聚糖或小肠结肠炎耶尔森菌全细胞刺激的多形核白细胞的CL反应。用链霉蛋白酶处理全细胞，去除质粒介导的OMP，导致全细胞和源自全细胞的OMP对CL的抑制部分但显著消除。与小肠结肠炎耶尔森菌孵育60分钟不影响多形核白细胞的活力。我们的结果表明，小肠结肠炎耶尔森菌与人类多形核白细胞的相互作用直接受质粒介导的OMP影响。
