Wall S J, Wolfe B B, Kromer L F
Department of Pharmacology, Georgetown University School of Medicine, Washington, District of Columbia 20007.
J Neurochem. 1994 Apr;62(4):1345-51. doi: 10.1046/j.1471-4159.1994.62041345.x.
Unilateral aspiration lesions of the rostral supracallosal stria/cingulum bundle and fimbria-fornix were performed on adult female rats. Ten and 24 days post lesioning, an elevation (17%; p < 0.01) of total muscarinic receptors was observed in lesioned versus control hippocampi. By using antisera selective for each of the five molecularly defined subtypes (m1-m5) of muscarinic receptors, significant changes were observed in the levels of expression for at least four receptor proteins. Three receptor subtypes increased in density: m1 by 14% (from 943 to 1,078 fmol/mg); m3 by 77% (from 150 to 268 fmol/mg); and m4 by 29% (from 220 to 285 fmol/mg). In contrast, a 22% decrease in the density of m2 receptors was found (from 220 to 173 fmol/mg). Detectable levels of m5 receptors were low in the hippocampus (approximately 1% of total receptors), and reliable measurements were not obtained. The directions of these changes are likely to be related to the pre- or postsynaptic localization of these receptor subtypes.
对成年雌性大鼠进行了胼胝体上缘纹/扣带束和穹窿-海马伞的单侧毁损性损伤。损伤后10天和24天，与对照海马相比，损伤海马中总的毒蕈碱受体升高（17%；p<0.01）。通过使用针对毒蕈碱受体五个分子定义亚型（m1-m5）中每一个亚型的抗血清，观察到至少四种受体蛋白表达水平有显著变化。三种受体亚型密度增加：m1增加14%（从943飞摩尔/毫克增至1078飞摩尔/毫克）；m3增加77%（从150飞摩尔/毫克增至268飞摩尔/毫克）；m4增加29%（从220飞摩尔/毫克增至285飞摩尔/毫克）。相比之下，发现m2受体密度降低22%（从220飞摩尔/毫克降至173飞摩尔/毫克）。海马中m5受体的可检测水平较低（约占总受体的1%），未获得可靠测量值。这些变化的方向可能与这些受体亚型的突触前或突触后定位有关。
