Larcombe-McDouall J B, Seo Y, Steward M C
School of Biological Sciences, University of Manchester, United Kingdom.
Magn Reson Med. 1994 Feb;31(2):131-8. doi: 10.1002/mrm.1910310206.
Changes in intracellular and extracellular water content have been measured in perfused rat salivary glands by repetitive application of an inversion recovery (IR) pulse sequence. The relaxation reagent Gd-DTPA (10 mM) was included in the perfusate so that the intracellular and extracellular water proton signals could be distinguished by their different longitudinal relaxation times. Changes in water content in response to altered perfusion pressure and perfusate osmolarity were determined at 30-s intervals and indicated a clear separation of the intracellular and extracellular components. Using a modification of the IR pulse sequence, changes in intracellular water content were also measured at 6-s intervals. With this time resolution, differences in the rates of cell shrinkage in response to hyperosmotic perfusates and the secretomotor agonist acetylcholine were observed. The results suggest that this approach offers a relatively noninvasive method for studying cell volume regulation in intact, perfused tissues and organs.
通过重复应用反转恢复（IR）脉冲序列，已对灌注大鼠唾液腺中的细胞内和细胞外含水量变化进行了测量。灌注液中加入了弛豫剂钆喷替酸葡甲胺（Gd-DTPA，10 mM），以便通过不同的纵向弛豫时间区分细胞内和细胞外水质子信号。以30秒的间隔测定了因灌注压力和灌注液渗透压改变而引起的含水量变化，结果显示细胞内和细胞外成分明显分离。使用改进的IR脉冲序列，还以6秒的间隔测量了细胞内含水量的变化。凭借这种时间分辨率，观察到了高渗灌注液和分泌运动激动剂乙酰胆碱引起的细胞收缩速率差异。结果表明，这种方法为研究完整灌注组织和器官中的细胞体积调节提供了一种相对非侵入性的方法。
