Samsonova A P, Anan'ina Iu V, Aksenov M Iu, Savel'eva O V, Gintsburg A L
Mol Gen Mikrobiol Virusol. 1994 Jan-Feb(1):19-23.
Polymerase chain reaction has for the first time been shown to be applicable to indication of Leptospira interrogans in the organs of infected animals with acute or chronic leptospirosis (on the model of golden syrian hamsters). Polymerase chain reaction is superior to microscopic and bacteriological analyses in identification of leptospirae in organ suspensions. The sensitivity of the technique is 1-10 cells per sample in studies of kidney or brain suspensions or 100-1000 cells in studies of liver suspensions.
首次证明聚合酶链反应可用于指示急性或慢性钩端螺旋体病感染动物器官中的问号钩端螺旋体（以金黄叙利亚仓鼠为模型）。在鉴定器官悬液中的钩端螺旋体方面，聚合酶链反应优于显微镜检查和细菌学分析。在肾脏或脑悬液研究中，该技术的灵敏度为每个样品1 - 10个细胞，在肝脏悬液研究中为100 - 1000个细胞。
