Anderson J F, Miller D A, Post J E, Johnson R C, Magnarelli L A, Andreadis T G
Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
J Am Vet Med Assoc. 1993 Dec 1;203(11):1550-1.
Leptospira interrogans serovar grippotyphosa was isolated from the skin of a 14-year-old male dog with deteriorating health. Necropsy revealed numerous lesions characteristic of aged dogs, but no evidence of acute hepatitis or nephritis, which are common features of pathogenic Leptospira infections. Antibody to Leptospira was not detected in the dog's serum by microagglutination. Leptospires grew slowly in Barbour-Stoenner-Kelly medium, a medium commonly used to isolate Borrelia, but then grew abundantly in Tween 80-bovine albumin leptospire medium. The isolate was pathogenic to a hamster and was identified by microagglutination and restriction endonuclease analysis.
从一只健康状况不断恶化的14岁雄性犬的皮肤中分离出了问号钩端螺旋体犬型流感血清型。尸检发现了许多老龄犬特有的病变，但没有急性肝炎或肾炎的迹象，而急性肝炎或肾炎是致病性钩端螺旋体感染的常见特征。通过微量凝集试验在该犬的血清中未检测到钩端螺旋体抗体。钩端螺旋体在常用于分离疏螺旋体的巴伯-斯托纳-凯利培养基中生长缓慢，但随后在吐温80-牛白蛋白钩端螺旋体培养基中大量生长。该分离株对仓鼠具有致病性，并通过微量凝集试验和限制性内切酶分析进行了鉴定。
