Pershin B B, Chakhava O V, Maganet L S, Novikova T A, Shustrova N M
Zh Mikrobiol Epidemiol Immunobiol. 1975 May(5):65-9.
Gnotobiological studies were conducted on germ-free guinea pigs. Association of the animals with the citrobacteria 5396/38 strain led to the reproduction of these bacteria in numbers exceeding the sum total number of all the representatives of the normal microbial flora in the conventional animals. Association lasting for a period of 46 days caused no antibody formation in the blood serum of these animals in difference to conventional guinea pigs immunized parenterally. Conventionalization of the monoflora guinea pigs served as the beginning of population of the intestinal tract by other microbial representatives which replaced the first culture. Biochemical properties of the citrobacteria strains isolated from the monoflora strains were identical to the properties of the initial culture. After the monoflora animals were let out of the isolation cage the citrobacteria strains displayed changes of some differential-diagnostic signs. Passage through the macroorganism of the citrobacteria stain proved to lead to the changes in the antigenic structure.
在无菌豚鼠身上进行了悉生生物学研究。将这些动物与柠檬酸杆菌5396/38菌株相接触，导致这些细菌大量繁殖，其数量超过了常规动物体内正常微生物群落所有代表的总数。持续46天的接触并未使这些动物的血清中形成抗体，这与经肠道外免疫的常规豚鼠不同。单菌豚鼠的常规化成为其他微生物代表在肠道中定殖的开端，这些微生物取代了最初的培养物。从单菌豚鼠中分离出的柠檬酸杆菌菌株的生化特性与初始培养物的特性相同。单菌豚鼠从隔离笼中放出后，柠檬酸杆菌菌株显示出一些鉴别诊断特征的变化。柠檬酸杆菌菌株通过大型生物体的传代证明会导致抗原结构的变化。
