Barber C
Microbios. 1981;32(128):71-6.
Immunochemical analysis of Citrobacter ballerup and Salmonella typhi Ty2 showed that the strains share native and heat-resistant proteins that are, apparently, the carriers of a common polysaccharidic determinant present in their respective somatic antigens. After the classic acetic acid hydrolysis, the somatic antigen of C. ballerup reacted, in agar gel, against the homologous antiserum by two precipitation lines, one of which also precipitated against the anti S, typhi Ty2 serum; the hydrolysis of the S. typhi Ty2 somatic antigen demonstrated that, in addition to the 'O' polysaccharide, reacting against all the S. typhi antisera, it contains a polysaccharide that precipitated against the anti-C. ballerup serum. The elusiveness in the agglutinability of only freshly isolated bacterial authorizes some doubt concerning the responsibility of the antipolysaccharide antibodies in the agglutinating Vi sera; in order to induce anitpolysaccharides hyperimmunizations are needed while antiproteins are easily induced by short immunizations.
对巴鲁普柠檬酸杆菌和伤寒沙门氏菌Ty2进行的免疫化学分析表明，这些菌株具有天然和耐热蛋白，显然，这些蛋白是它们各自菌体抗原中存在的共同多糖决定簇的载体。经过经典的乙酸水解后，巴鲁普柠檬酸杆菌的菌体抗原在琼脂凝胶中与同源抗血清通过两条沉淀线发生反应，其中一条沉淀线也能与抗伤寒沙门氏菌Ty2血清发生沉淀反应；伤寒沙门氏菌Ty2菌体抗原的水解表明，除了与所有伤寒沙门氏菌抗血清发生反应的“O”多糖外，它还含有一种能与抗巴鲁普柠檬酸杆菌血清发生沉淀反应的多糖。仅新鲜分离细菌的凝集性难以捉摸，这使得人们对凝集Vi血清中抗多糖抗体的作用产生了一些怀疑；为了诱导产生抗多糖，需要进行超免疫，而抗蛋白则很容易通过短期免疫诱导产生。
